200ASSESSMENT OF IN VITRO ANTI-DIABETIC PROPERTIES OF CATUNAREGAM SPINOSA EXTRACTSDiabetes mellitus (DM) is a significant metabolic condition marked by persistent high blood glucose levels, often leading to severe complications and an increasing global health burden. Traditional treatments, while effective, can be costly and may result in side effects, creating a demand for alternative, affordable, and safer therapies. Catunaregam spinosa, a plant used in traditional medicine, has gained attention for its potential antidiabetic properties. This study explores the in vitro antidiabetic potential of aqueous and ethanolic extracts of C. spinosa by evaluating their inhibitory effects on essential digestive enzymes, ?-amylase and ?-glucosidase, which are crucial in postprandial blood glucose regulation. The ethanolic extract exhibited stronger inhibitory effects on both enzymes, with IC?? values of 225.81 ± 4.11 ?g/mL for ?-amylase & 211.67 ± 2.12 ?g/mL for ?-glucosidase, compared to the aqueous extract. These results suggest that C. spinosa could serve as a promising natural source of enzyme inhibitors, potentially beneficial in managing diabetes. Additional research is needed to isolate and characterize the specific bioactive compounds responsible for these effects and to confirm their efficacy through in vivo studiesvaram9@gmail.comResearch622016Catunaregam spinosa, Antidiabetic activity, ?-amylase inhibition, ?-glucosidase inhibition, Plant-based therapyDr. Prasad Mettikolla*1 and G. Sangeetha*1Independent Researcher, Shri Balaji Drugs INC, 8808 Camp Bowie West Blvd, Fort Worth, TX-76116, USA. 2Krupanidhi College of Pharmacy, 12/1, Chikka Bellandur, Varthur Hobli, Bangalore – 560 035, Karnataka, INDIA