184A STRONGER ANTI-AMOEBIC EFFECT ON ENTAMOEBA HISTOLYTICA: HETEROPHRAGMA ADENOPHYLLUMThis plant belongs to the family Bignoniaceae as a synonym of Fernandoa adenophylla, which is also known as Katsagon or Marodphali. It is used clinically for antidiabetes, amenorrhea, premature ejaculation, night emesis, anti-microbials, antifungals, antiseptics, and skin diseases. In contrast, the effect of H. adenophyllum on Entamoeba histolytica, the causative agent of amoebiasis,is not known . Ethanolic extracts from aerial parts, roots, and roots ethyl acetate extracts were experimented on E. histolytica trophozoites. A comparison of HAEEA and HAEER showed no anti-proliferative effect, but HAEER demonstrated an in vitro GI50 of 225 milligrams/ml and inhibited the differentiation of E. invadens trophozoites into cysts. Hamsters infected with E. histolytica were given HAEAR (2.5 or 5 mg) intraperitoneally, and the drug inhibited the development of amoebic liver abscesses in 45.56 or 85.0% of the animals, respectively. Adhesion to fibronectin and erythrophagocytosis were 27.77 and 47.01% inhibited by HAEAR, respectively. Our results suggest that HAEAR may be effective in treatments against amoebiasis, nevertheless, detailed toxicity studies on thiophenes, contained in HAEAR, are required to avoid misuse of this vegetal speciesdrujwalasunkanapallyy@gmail.comResearch1312023Actin,Heterophragma adenophyllum,AmoebiasisSunkanapally Ujwala,Purushothaman MKLR Pharmacy College, Paloncha, Telangana-507115, India,KLR Pharmacy College, Paloncha, Telangana-507115, India